恳请大家帮忙，给我修改一下简历

ssssss

来澳洲一个多月了，从一月十日开始，投了大概40份简历，今天收到刚开始投的那些单位的拒信。简历修改了几次，现将当前的版本贴出来，希望大家帮忙修改一下，在seek上看，我的相关的工作比如Medical Scientist，laboratory Scientist，和research assistant还是比较多的，有的职位很适合我，但至今也没有收到消息，可能第一与closing day 还没到有关，另外就是可能是自己不合适吧。昨天去Monash medical research center和Gribble pathology投了简历，目标是：A 医院的化验室偏重分子生物学检验如病毒载量的测定，基因分型，PCR,RT-PCR等，在这边就是pathology，B 研究所如ludwig，WEHI,BURNET 传染病研究所等。本人专业技术过硬，凡是简历所列的，都做过很多次。恳请大家帮忙改一下简历，希望自己早日找到工作。没工作的感觉好难受，目前在一个包装厂打casual job，收入很少，不能cover 生活费。
简历如下：                                                            

Sophia Z

E-mail:xxxxxxxxxxx@gmail.com 　　　　　　　　　　　　　　　　　XX  xxxxx Crescent

Tel: 000000000　　　　　　　　　　　　　　　　　　　　　Dandenongl North, VIC 3175　　　　　　　　　　　　　　　　　　　　　　　

CAREER OBJECTIVE
·Life Science Professional in medical research institute ,medical laboratory or company
with more than 5 years of practical experience and theoretical training
·An effective team player that is proficient in research experimental methods and feasibility research with the ability to be extremely productive in an ever changing environment
·The opportunity of performance-based advancement

CORE STRENGTHS
·Basic and advanced molecular biology and cell biology laboratory skills
·Broad knowledge of biology-related topics, with a strong focus on bioinformatics
·Proficient in many software packages, both standard desktop as well as scientific
·Quickly learning new knowledge and skills
·Details oriented and restless pursuit of creative goals
·Good analytical skills and perfectly able to work under stress.
·Excellent writing and documenting skills.


SKILLS MATRIX

Classification	Details
Project Performance	lSubscribing to and keeping experimental agents and consumbles lPerforming ordinary laboratory experiments lDocumenting the entire experimental details, procedures and outcomes lReporting and discussing the results to the laboratory director or senior research staff lCollecting related scientific data and analysing the experimental results lWriting research papers and contacting potential publishers
Applications	lBioinformatics: 1.Running and maintenance of ABI3100 automated DNA sequencer 2.Performing resistance genotyping using the viroseq HIV-1 Genotyping Kit 3.Performing HIV-1 pol and vpr gene genotyping 4.One of my research was titled Genetyping and Molecular Characterization of HIV-1 vpr gene in Hunan Province, China 5.Sequence analysis using bioinformatics techniques. Analysis is done using the following software: Clustalw, PAUP, TREETOOL , BIOEDIT, GENEDOC, TREEVIEW, MEGA lMolecule biology: DNA and RNA isolation, PCR, RT-PCR, agarose gel electrophoresis, sequencing of PCR, genotyping of viruse such as HIV, DNA recombination, plasmid/retroviral plasmid construction including preparing culture media, making competent cells such as from JM109, enzyme cut and ligation, bacteria culture, plating and evaluation, subcloning, stable/transient transfection by Lipofectamine, FuGene 6, retrovirus, Nucleofector and Electroporation, siRNA, antisense, situ hybridization, Southern and Northern transfer lCell biology: Cell lines/primary cell culture, cell growth curve, cell apoptosis and differentiation assays, immunofluorescent double/triple staining, cell sorting by FACS, protein extraction and purification, stably expressing protein cell lines lMicrobiology: sample and media preparation, their disposal plating of samples,reconstructed plasmid through enzyme cut and ligation. lImmunology: Primary T cell isolation, culture and transfection, luciferase assay, ELISA, immunoprecipitation, Western blotting, immunocytochemistry, immunohistochemistry, flow cytometry
Tools	lProficiency in: 1.MS OFFICE (Excel, Word, PowerPoint, Project, Outlook) 2.Biology software Primer 3, ClustalW, Sigmaplot ,GeneDoc and BLAST 3.A large number of population and evolutionary genetics packages such as PAUP, MEGA , DNAsp and others 4.Molecular biology and medical databases (e.g. NCBI, UCSC, Ensembl, EMBL, HapMap and TSC) 5.Statistical Package:SPSS,Origin7.0 lSkilled in: PCR Instrument, Centrifugal Machine, electrophoresis apparatus, Gel Imagine System, Biological safety cabinets and Cell Culture System
Operating System	lWindows series
Industry Experience	lMedical Laboratory, Research laboratory and Clinical of internal Medicine

Professional Experience XX  Research Laboratory, XXX South University  September
2003~July 2008
Life Scientist
Responsibilities
·Subscribed to and kept experimental agents
·Performed ordinary laboratory experiments which were assigned and documented details and procedures
·Reported and discussed the results to the laboratory director
·Collected related scientific data and analysed the experimental results
·Wrote research papers and contacted potential publishers
·Worked closely with laboratory teams to ensure that functional and technical solutions were aligned to research project requirements.
·Kept abreast of advancements in life science, controlled the project implementation schedule, and participated in managing issues, risk and change.

Major projects:

Project name: The HIV-1 Vpr Genetic Diversity and Functional Features and its potential mutation among the long non-progressing patients among the long non-progressing patients, supported by the National Natural Science Foundation of China
Principle Investigator
·Assessed the progress of the experimental implementation project, and created a plan to ensure the successful finalization of the experiment implementation
·Took responsibility for management of the main tasks and the project unwinding, in coordination with related field experts
·Paid close attention to the experimental details in order to minimize the waste of time and agents
·Performed almost all procedures including
1.Constructed phylogenetic trees and analyzed HIV-1 genetic diversity:
preparation samples, HIV-1 RNA(from plasma) or HIV-1 genome(DNA from lymphocyte) extraction, nested RT-PCR, PCR sample sequenced, alignment, subtyping, constructed the phylogenetic trees, analyzed features of genetic diversity.
2.The role of reconstructed vector pcDNA-vpr on apoptosis:
Selected the representative mutant, reconstructed plasmids through enzyme cut and ligation, transformed into competent cells JM109, transfected into CD4+ cells by liposome transiently, harvested after 72 hours with G418 selective medium, mRNA expression was detected by RT-PCR, cell viability detected by typan blue staining methods, fluorescent microscope observed Hoeschst stained cell for death cells, percentage of apoptosis measured by flow cytometry using Anexcin-FITC-PI staining and DNA fragmentation was analyzed by agarose gel electrophoresis
Achievement:
1.Performed as a both technician and scientist to effectively had gotten the project go on smoothingly
2.Successfully finished the project
3.Fully documented the entire experimental cycle in order to make necessary experimental regulation.

XXX Hospital, China, (Jul 2001 ~ Jul2003)
Resident of Internal Medicine Department
Responsibilities &Achievement
·Gained extensive hands-on experience in basic general medicine as well as the intricacies of subspecialty internal medicine, including training in both outpatient
·Took on significant responsibility in making decisions about the care of patients

Publications（４篇，给出了杂志名，和链接地址）

Education
XXX Medical College 　　　　　　　　　　　
September/1996~July/2001
Bachelor of Medicine

Referees  （３名）



[ 本帖最后由 ssssss 于 8-2-2009 17:19 编辑 ]

helenazy

CAREER OBJECTIVE和CORE STRENGTHS有没有无所谓.

SKILLS MATRIX ;

给你提示，什么东西都要说明用了多久，洋人最讲究精确了．５年经历和３年的工资差老多来！

Tools：建议你放最前面，洋人最关心工具了．
用　XX years in MS OFFICE (Excel, Word, PowerPoint, Project, Outlook)
Project Performance: 你放具体项目里讲好了,这里可要可不要，建议不要放了
Appliaction
lBioinformatics:
我举个例子, 你把人家关心的项目放前面去好吧:
1.Running and maintenance of ABI3100 automated DNA sequencer----->
XX years in ABI3100 automated DNA sequencer Running and maintenance
理解？

lMolecule biology: 我看了彻底昏了，估计中介也不高兴看的，你列几个重点好吧，研究研究人家广告，找热门的列．

后面的长度差不多，但改成XX years in CC

Professional Experience

我看了快晕倒了！！！
Responsibilities，　不要写在开头，具体到后面的每个项目里去！！！！！
Major projects: 项目名字写成一句话，这是名字么？
Principle Investigator：你写太多了，这是简历！！！！！！

通常是：
你举一个项目太少，举两三个差不多
Project name:
Job Title
Project background: brief introduce
Responsibilities:
Achievement: 吹吹你具体干了什么，但记住，这是简历


XXX Hospital, China,　你好歹也写点什么吧，这两年白呆了，格式见上．就算白呆，你也得编点东西出来．

最后，推荐人写，available if request，别写具体联系方法．真要问，你可以先去打招呼．

ssssss

SKILLS MATRIX

Classification	Details
Tools	lSkilled in: PCR Instrument, Centrifugal Machine, electrophoresis apparatus, Gel Imagine System, Biological safety cabinets and Cell Culture System lProficiency in: 1.MS OFFICE (Excel, Word, PowerPoint, Project, Outlook) 2.Biology software Primer 3, ClustalW, Sigmaplot ,GeneDoc and BLAST 3.A large number of population and evolutionary genetics packages such as PAUP, MEGA , DNAsp and others 4.Molecular biology and medical databases (e.g. NCBI, UCSC, Ensembl, EMBL, HapMap and TSC) Statistical Package:SPSS,Origin7.0
Applications	lBioinformatics: 1.Performing resistance genotyping using the viroseq HIV-1 Genotyping Kit 2.Performing HIV-1 pol and vpr gene genotyping 3.Sequence analysis using bioinformatics techniques. Analysis is done using the following software: Clustalw, PAUP, TREETOOL , BIOEDIT, GENEDOC, TREEVIEW, MEGA lMolecule biology: DNA and RNA isolation, PCR, RT-PCR, agarose gel electrophoresis, sequencing of PCR, DNA recombination. lCell biology: Cell lines/primary cell culture, cell growth curve, cell apoptosis and differentiation assays, immunofluorescent double/triple staining, cell sorting by FACS, protein extraction and purification, stably expressing protein cell lines lMicrobiology: sample and media preparation, their disposal plating of samples, reconstructed plasmid through enzyme cut and ligation. lImmunology: Primary T cell isolation, culture and transfection, luciferase assay, ELISA, immunoprecipitation, Western blotting, immunocytochemistry, immunohistochemistry, flow cytometry lPathology: techniques for sample/section preparation and HE staining, immunohistological double/triple staining
Operating System	lWindows series
Industry Experience	lMedical Laboratory, Research laboratory and Clinical of internal Medicine

Professional Experience
xxxx Research Laboratory, XXX  University ,September
2003~July 2008
Major projects:

Project name:The HIV-1 Vpr Genetic Diversity and Functional Features
Job Title: Research assistant
Project Background:The viral protein R (Vpr) of humam immunodeficiency virus type-1(HIV-1) is a small basic protein (14 kDa) of 96 amino acids, and is well conserved in HIV-1, HIV-2 and SIV. The role of Vpr in the pathogenesis of AIDS is undeniable, but its genetic diversity and real functions during the natural course of infection are still subject to debate.
Responsibilities:
·Assessed the progress of the experimental implementation project, and created a plan to ensure the successful finalization of the experiment implementation
·Performed almost all procedures including RNA/DNA isolation, RT-PCR, sequence , constructed phylogenetic trees and analyzed HIV-1 vpr genetic diversity
Project name: The role of mutated vpr gene on CD4+ cell
Job Title: Research Assistant
Project Background:
The capability of Vpr induced apoptosis when its amino acid occurred mutation.
Responsibilities:
1.Reconstructed vector pcDNA-vpr
2.Transfected into CD4+ cells by liposomemRNA expression was detected by RT-PCR, cell viability detected by typan blue staining methods, fluorescent microscope observed Hoeschst stained cell for death cells, percentage of apoptosis measured by flow cytometry using Anexcin-FITC-PI staining and DNA fragmentation was analyzed by agarose gel electrophoresis
Achievement:
1.Performed as a both technician and scientist to effectively had gotten the project go on smoothingly
2.Successfully finished the project
3.Fully documented the entire experimental cycle in order to make necessary experimental regulation.

xxx  Hospital, China, Jul 2001 ~ Jul2003
Resident of Internal Medicine Department
Responsibilities &Achievement
·Gained extensive hands-on experience in basic general medicine as well as the intricacies of subspecialty internal medicine, including training in both outpatient
·Took on significant responsibility in making decisions about the care of patients

Publications

Education
xxx Medical College , China
September/1996~July/2001
Bachelor of Medicine

Referees available upon request

已经按照helen版主的要求修改了一下，麻烦再看一下。
1 去掉了career objective和core strenths
2 因为这些仪器和技术是序贯交叉使用的，所以各自的年限无法计算。
3 将tool放在前面，删掉molecular的一些内容
4 在hospital的经历，没有project，我再想想还有什么可写的东西。

[ 本帖最后由 ssssss 于 6-2-2009 23:35 编辑 ]

helenazy

看起来干净很多了，不过问题依然不小。你考虑下你的职业定位，实验室做的，报告肯定得天天写吧？E文简历是敲门砖，这个首先得花工夫搞得好啊！

你别太强调理由，使用工具和技术年份你自己毛估估下就是了，交叉使用么，就往多了写了。只要4年中，每一年有一天用到过，就算四年。你不写不要怪我没说，实验室工作尤其讲究数字，你连简历上都不给人家几个数字，自己想想，说服力够强么？

Major projects:

Project name:The HIV-1 Vpr Genetic Diversity and Functional Features （请考虑下语法问题，翻译成中文都不通的呀，‘HIV-1 Vpr基因离散和功能特征’，这是什么，说明书开头还差不多，有关于‘HIV-1 Vpr基因离散和功能特征’的研究（research之类的），才是正解释）
Job Title: Research assistant
Project Background:The viral protein R (Vpr) of humam immunodeficiency virus type-1(HIV-1) is a small basic protein (14 kDa) of 96 amino acids, and is well conserved in HIV-1, HIV-2 and SIV. The role of Vpr in the pathogenesis of AIDS is undeniable, but its genetic diversity and real functions during the natural course of infection are still subject to debate.（那你这项目的目的是什么，请说说清楚．This project is set up to.....）
Responsibilities:（是你的责任，不是这项目的责任，要么你把他改成‘成就’，但动名词还是用得不对）
·Assessed（Accessing） the progress of the experimental implementation project, and created a plan to ensure the successful finalization of the experiment implementation(大人啊，你做研究，你是主动的，动名词用现在分词，而不是过去分词，后面是差不多毛病)
·Performed almost all procedures including RNA/DNA isolation, RT-PCR, sequence , constructed phylogenetic trees and analyzed HIV-1 vpr genetic diversity


Project name: The role of mutated vpr gene on CD4+ cell
Job Title: Research Assistant
Project Background:
The capability of Vpr induced apoptosis when its amino acid occurred mutation.（这什么意思，没头没尾的，简历写作态度不正确）
Responsibilities::（是你的责任，不是这项目的责任）
1.Reconstructed vector pcDNA-vpr
2.Transfected into CD4+ cells by liposomemRNA expression was detected by RT-PCR, cell viability detected by typan blue staining methods, fluorescent microscope observed Hoeschst stained cell for death cells, percentage of apoptosis measured by flow cytometry using Anexcin-FITC-PI staining and DNA fragmentation was analyzed by agarose gel electrophoresis
Achievement:
1.Performed as a both technician and scientist to effectively had gotten the project go on smoothingly
2.Successfully finished the project（这能算成就，这是基本职业要求，要么你写提前完成任务几个月）
3.Fully documented the entire experimental cycle in order to make necessary experimental regulation.（这能算成就，这是基本职业要求，要么你写实验报告上了啥啥国际权威杂志）

xxx  Hospital, China, Jul 2001 ~ Jul2003
Resident of Internal Medicine Department
Responsibilities &Achievement
·Gained extensive hands-on experience in basic general medicine as well as the intricacies of subspecialty internal medicine, including training in both outpatient
·Took on significant responsibility in making decisions about the care of patients
你编点内容出来吧，比如说，处理了多少临床案例，数字数字！！！

ssssss

非常感谢helen版主，明天继续修改。

ssssss

SKILLS MATRIX

Classification	Details
Tools	lSkilled in (5 years): PCR Instrument, High speed Centrifuge, electrophoresis apparatus, Gel Imagine System, Biological safety cabinets ,Cell Culture System, Microscope lProficiency in (5 years): 1.MS OFFICE (Excel, Word, PowerPoint, Project, Outlook) 2.Biology software Primer 3, ClustalW, Sigmaplot ,GeneDoc and BLAST 3.A large number of population and evolutionary genetics packages such as PAUP, MEGA , DNAsp and others 4.Molecular biology and medical databases (e.g. NCBI, UCSC, Ensembl, EMBL, HapMap and TSC) 5.Statistical Package:SPSS,Origin7.0
Applications	lBioinformatics(2 years): 1.Performing resistance genotyping using the viroseq HIV-1 Genotyping Kit 2.Performing HIV-1 pol and vpr gene genotyping 3.Sequence analysis using bioinformatics techniques. Analysis is done using the following software: Clustalw, PAUP, TREETOOL , BIOEDIT, GENEDOC, TREEVIEW, MEGA lMolecule biology (5 years): DNA and RNA isolation, PCR, RT-PCR, FQ-PCR, agarose gel electrophoresis, sequencing. lCell biology(4 years): Cell lines/primary cell culture, cell growth curve, cell apoptosis and differentiation assays, immunofluorescent double/triple staining, cell sorting by FACS, protein extraction and purification, stably expressing protein cell lines lMicrobiology (1 year): sample and media preparation, plating, plasmid reconstruction. lImmunology(5 years): Primary T cell isolation, culture and transfection, luciferase assay, ELISA, immunoprecipitation, Western blotting, immunocytochemistry, immunohistochemistry, flow cytometry lPathology(6 months): techniques for sample/section preparation and HE staining, immunohistological double/triple staining
Operating System	lWindows series
Industry Experience	lMedical Laboratory(2 years), Research laboratory(3 years) and Clinical Internal Medicine as a resident doctor(2.5 years)

Professional Experience

Research Laboratory, aaa University (Sep
2003~Jul 2008)

Major projects:

Project name:HIV-1 vpr gene diversity detection and its correlation with clinical characteristics of AIDS
Job Title: Research assistant
Project Background:This project is set up to explore genetic diversity and molecular epidemic characteristics of HIV-1 vpr gene among Chinese HIV-1 infected population. The viral protein R (Vpr) of humam immunodeficiency virus type-1(HIV-1) is a small basic protein (14 kDa) of 96 amino acids, and is well conserved in HIV-1, HIV-2 and SIV. The role of Vpr in the pathogenesis of AIDS is undeniable, but its genetic diversity and real functions during the natural course of infection are still subject to debate.
Responsibilities:
·Extracting viral RNA from plasma samples
of HIV-1 infected patients
·Reversely transcribing RNA into DNA by RT-PCR and amplified by nested PCR
·Purifying and sequenced amplified DNA fragments
·Aligning DNA sequences with known vpr representative sequence available in the Las Alamos database
·Using DNAsp software to analyze the polymorphism of nucleotides of vpr gene
·Collecting related clinical and scientific data
·Analyzing the experimental results and discussed the results to the laboratory director or senior research staff
·Writing research papers and contacting potential publishers
Achievement:
·Assessed the progress of the experimental implementation project
·Created a plan to ensure the successful finalization of the experiment implementation
·Performed almost all experimental procedures efficiently
·Completed the project on time and saved budget 20%

Project name: The role of the HIV-1 mutated Vpr Protein in the Modulation of the Apoptotic Response of T Cells
Job Title: Research Assistant
Project Background:
This project is set up to investigate the role of mutated Vpr protein in regulating apoptosis.
Responsibilities:
1.Ordering supplies and ensuring general laboratory safety and cleanliness
2.Documenting the entire experimental details, procedures and outcomes
3.Reporting and discussing the results to the laboratory director or senior research staff
4.Performing mammalian cell culture and setting up the cell line
5.Performing the cell apoptosis assays by flow cytometry using Anexcin-FITC-PI double staining, DNA fragmentation and Hoeschst staining.
Achievement:
1.Performed as a both technician and scientist in a professional manner
2.Fully documented the entire experimental cycle in order to comply with the necessary experimental regulations.
3.Successfully completed three months ahead of schedule

XXX Hospital, China(Jul 2001 ~ Jul2003)

Job title: Resident Doctor of Internal Medicine Department
Responsibilities:
1.Performing the initial assessment of the patient and actively participate in all aspects of patient care, including history and physical, diagnostic and therapeutic planning, procedures, writing orders, and interactions with family.
2.In-depth discussion of all cases with the attending prior to initiation of all but the most basic diagnostic studies or therapeutic interventions.
3.Maintaining medical records.
Achievement:
1.Rotating 6 main internal medicine departments as following (each department for 2 months) : cardiology department , neurology department , nephrology department , respiratory medicine , gastrointestinology department ,hematology department, and specialized in infectious disease division(1.5 years)
2.Gained extensive hands-on experience in basic general medicine as well as the intricacies of subspecialty internal medicine, including training in both outpatient
3.Tackling with about totally 750 inpatients of the above departments

Publications

Education
XXX Medical College , China
September/1996~July/2001
Bachelor of Medicine

Referees available upon request

[ 本帖最后由 ssssss 于 8-2-2009 17:17 编辑 ]

ritz

I would suggest that not giving out your email and phone numbers here, it may cause some unwelcome troubles

ssssss

按照helen版主的建议，进行了第二次修改。麻烦再给看一下。非常感谢！

helenazy

看了，从我的角度来看，象样太多了．

不过，Achievement和Responsibilities里动名词依然没统一好．比如说这里，还有别的地方，一堆ＥＤ！！！

·Analyzing the experimental results and discussed the results to the laboratory director or senior research staff